Patients were admitted to the hospital the day before and bowel
prep administered the night before FMT. Patients were also
administered a proton pump inhibitor (omeprazole, 20 mg) on the
evening and morning before the procedure. Donor fecal samples
(25–30 g) were mixed with 250 ml of sterile saline buffer, mixed
into slurry and filtered once with surgical gauze for large particles
and twice with a coffee filter. The volume of the filtrate was
increased to 450 ml with sterile saline buffer and divided into 5
aliquots of 90 ml. For FMT, two aliquots (180 ml) were
endoscopically delivered by spray catheter into the jejunum. The
remaining three aliquots were instilled by colonoscopy into the
right colon (180 ml) and transverse and upper descending colon
(90 ml).