In the left horn of group 2, five days after injection of clove essence, complete necrosis of horn bud with a few neutrophils infiltrated around the necrotic tissues was observed. The epidermis, over the horn buds showed necrosis and ulceration. There was a mild infiltration of neutrophils in the surface of necrotic epidermis. Necrotic tissues and degenerated neutrophils formed scab on the ulcerated area. Underlying dermal tissues revealed necrosis and collagenolysis (Fig. 4).