The global significance of 1RS in wheat breeding programs has been well-reviewed.
Baum and Appels (1991) called chromosome 1R ‘one of the most widely utilized sources of alien chromatin in wheat cultivars’ (Berzonsky and Francki, 1999). This alien segment has significantly contributed to enhance the agronomic performance, particularly grain yield and environmental stability. Hence,wheat breeders worldwide have used the T1BL.1RS germplasms in their breeding programs (Villareal et al., 1997). To use the T1BL.1RS germplasms for wheat improvement, complete characterization of the
introgressed segment, determination of the wheat chromosome arm involved in translocation, source of rye chromatin, and the amount of 1RS chromatin introduced are
necessary. Cytogenetics, molecular, and biochemical technologies have been reported
which enable such a characterization of 1RS in wheat (Berzonsky and Francki, 1999). It is hence crucial that native wheats to Pakistan should be diagnosed for this exchanged
genetic material. Hence, the present study was designed to analyze the presence of T1BL.1RS translocation in Pakistani wheat cultivars and candidate lines for future cultivars in the country.