MDA concentration was determined according to the methods described byHartman et al. (2004). Fresh leaves in 0.5 g
were homogenized in a solution containing 0.6% thiobarbituric
acid (TBA) and 5% trichloroacetic acid (TCA), heated at 100
◦
C for
25 min and centrifuged at 8000×gfor 10 min after cooled to room
temperature.