Group 1 was the control group fed with balanced diet. At the beginning, blood samples were collected from group 1 only and transferred to the lab. The remaining five groups (group 2, group 3 and 0.1, 0.2, 0.3 % garlic) were fed with high fat diet prepared from plate and animal fat with 3 to 1 ratio, for 14 days. Afterwards, blood sampling was performed on group 2 and transferred to the lab and the group was excluded.
The other four group received their feeding for 7 days as follows: group 3 received probiotic Lactobacillus acidophilus milk without garlic, group 4 received probiotic Lactobacillus acidophilus milk with 0.1% garlic, group 5 received probiotic Lactobacillus acidophilus milk with 0.2% garlic and finally group 6 received the same milk but with 0.3% garlic.
During this period, 30 ml/day of milk was given to each rat and blood sampling was performed on the four remaining groups and transferred to the lab.