Spermatological analysis was performed at the Dadman
International Sturgeon Research Institute, Rasht, Iran. For
each treatment, 32 male broodstock were selected. The
semen was prepared 22 hours after hormone injection.During semen collection, the anal fins of the broodstock
were dried completely. The semen was stripped by
abdominal massage, collected into glass vials, and stored on
ice until ready for use. Fresh sperm was immediately
maintained at 4 C, and the sperm concentration was
measured using a Neubauer chamber hemocytometer and
the following method: duplicate samples were prepared
for each semen sample, and a triplicate count was made on
a hemocytometer for each dilution.