Breads were baked using a commercial bread maker SD-BH103
(Panasonic Co., Osaka, Japan). The baking formula followed the
supplier’s recommendation, i.e., rice flour (300 g), distilled water
(230 g), salt (5.0 g), sugar (9.0 g), and olive oil (10 g). Proteases were
added based on their activity so that all were equal in terms of
activity. The ingredients were mixed by kneading with paddles in
the bread maker for 5 min. A formulation without any enzyme was
used as a control. After incubating the batter at 25 C for 16 h, the
batter was mixed by kneading with paddles in the bread maker for
5 min. The instant dried yeast (4.2 g) was then added to the batter.
The batter was mixed, proofed for 10 min, kneaded for 25 min,
fermented for 35 min, and baked for 40 min according to the
supplier’s protocol for rice bread making.