2.1.2. Inoculums for composting
The high-performance mixed flora containing microorganisms that could decompose starch, glucose, and protein had been isolated in a previous study (Yang et al., 2013) and preserved at below −80 °C in glycerol. The high-performance mixed flora was activated in Luria–Bertani culture medium (Miller, 1992) before the composting systems were inoculated with them. The concentration of the high-performance mixed flora seed that was used was >1 × 1015 colony forming unit (CFU) mL−1.