Gel and blot analysis of DNA from transformants.
Plasmid pHGART and various cell-line genomic DNAs (6 ,ug) were
digested with BamHI and electrophoresed on a 0.7% agarose gel.
(Left) Photograph of gel stained with ethidium bromide. (Center)
Autoradiograph after blotting to a nitrocellulose filter, probing with
the 3.9-kb BamHI Gart locus fragment containing all or part of six
exons (Fig. 1), and exposing for 15 min. (Right) Same as Center
exposed for 24 hr. The band detected is 3.9 kb in length.