Brackishwater aquaculture practices involve growing shrimp in high densities using feeds rich in nitrogen. The biogeochemistry of nitrogen in shrimp culture ponds is dominated by biological transformations of organic and inorganic nitrogen. Toxic forms of nitrogen viz. ammonia and nitrite are converted to nitrate and nitrogen gas through nitrification and denitrification processes. Denitrification and anammox are the key microbial processes responsible for the removal of fixed nitrogen from wastewater through the production of dinitrogen from wastewater through the production of dinitrogen.
Denitrification is a respiratory process in which nitrate and nitrite are converted into gaseous nitrogen intermediates viz. nitric oxide, nitrous oxide and finally nitrogen through the action of enzymes viz. nitrate reductase, nitrite reductase, nitric oxide reductase and nitrous oxide reductase. Denitrification is said to be ubiquitous in most aquatic sediments and is considered an important process of ecological significance since it permanently removes nitrogen from a system that would otherwise be available for primary production. In aquaculture ponds, denitrification takes place in the sediments, due to the presence of anoxic conditions and through degradation of organic matter. Although denitrification has been intense studied at the process level, less is known on the microbial species composition. Distribution, and functional dynamics.
Materials and methods
Sampling
Sediment samples used for this study were collected during august September from brackishwater shrimp culture pounds located in tamil nadu and andha Pradesh on the east coast and kerala and Gujarat on the west coat of india. Seven of these farms practiced traditional shrimp culture. The culture period in these farms varied from day to110 days and and the salinity ranged from 10 to 35 ppt. composite core samples of sediments was collected using sterile PVC corers as described by , in ice box , refrigerated on arrival and processed with in 4
Brackishwater aquaculture practices involve growing shrimp in high densities using feeds rich in nitrogen. The biogeochemistry of nitrogen in shrimp culture ponds is dominated by biological transformations of organic and inorganic nitrogen. Toxic forms of nitrogen viz. ammonia and nitrite are converted to nitrate and nitrogen gas through nitrification and denitrification processes. Denitrification and anammox are the key microbial processes responsible for the removal of fixed nitrogen from wastewater through the production of dinitrogen from wastewater through the production of dinitrogen.Denitrification is a respiratory process in which nitrate and nitrite are converted into gaseous nitrogen intermediates viz. nitric oxide, nitrous oxide and finally nitrogen through the action of enzymes viz. nitrate reductase, nitrite reductase, nitric oxide reductase and nitrous oxide reductase. Denitrification is said to be ubiquitous in most aquatic sediments and is considered an important process of ecological significance since it permanently removes nitrogen from a system that would otherwise be available for primary production. In aquaculture ponds, denitrification takes place in the sediments, due to the presence of anoxic conditions and through degradation of organic matter. Although denitrification has been intense studied at the process level, less is known on the microbial species composition. Distribution, and functional dynamics.Materials and methodsSamplingSediment samples used for this study were collected during august September from brackishwater shrimp culture pounds located in tamil nadu and andha Pradesh on the east coast and kerala and Gujarat on the west coat of india. Seven of these farms practiced traditional shrimp culture. The culture period in these farms varied from day to110 days and and the salinity ranged from 10 to 35 ppt. composite core samples of sediments was collected using sterile PVC corers as described by , in ice box , refrigerated on arrival and processed with in 4
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