2. Materials and methods
2.1. Materials
Highly purified SEA (purity > 95% as indicated on the product
specification sheet) was obtained from Toxin Technology Inc. (Sar-
asota, USA). The lyophilised toxin provided was reconstituted with
water following the instructions of the material provider, and fur-
ther dilutions were made using the following buffer: 10 mM NaH2-
PO4 dodecahydrate, 145 mMNaCl, adjusted to pH 7.3 with HCl, and
0.2% (w/v) bovine serum albumin (BSA). Raw milk cheese of the
type ‘‘Tomme de Savoie’’ with 28% fat content was obtained from
Coopérative Laitiere de Yenne Porte de Savoie, Yenne, France.