alpha (20 ng/ml; Fig. 6A). With EMSA gel shift assay, plaunotol-activated NF-κB was bound to DNA, similar to that stimulated by TNF-alpha (Fig. 6B).
Moreover, in the serum-starved RGM1 cells treated with plaunotol for 15 min, the phosphorylation of CRE was also increased compared to that of controls (Fig. 7).