In addition there are very few reports for D-dimer determination using biosensors based on electrochemical (Chebil et al., 2010), magnetoresistive (Gao et al.,2015) or impedimetric detection (Bourigua et al., 2010) from which only the latter is label-free. In all three cases, a limited number of samples have been employed for the evaluation of the sensor and a moderate matrix effect was observed. In this work we present for the first time in the literature, at least to our knowledge, the simultaneous determination of C-reactive protein (CRP) and D-dimer in human plasma samples.