Nowadays, many boar reproduction researches are directed to improve extenders and to increase cold shockprotection of semen. Little research is focused on the influence of egg yolk combined with alternative cold shockprotective media. Egg yolk could interfere with other compounds present in the extender composition. The influenceof egg yolk addition was assessed in boar sperm cells, cooled at 5ºC, to elucidate its effect on motility and membraneintegrity. Flow Cytometry and Computer Assisted Semen Analysis (CASA) were used to determine the rate of spermwith intact plasma and acrosomal membrane, respectively the sperm cells motility. Statistical analyses (T-Test) wereperformed using GraphPad Prism version 5.00. Androhep Plus supplemented with 20% egg yolk (AhPlus+20%EY)indicated a higher cold shock protection in progressive motility (93.9±2.64%) and membrane integrity(79.78±4.14%), rather than the extender without egg yolk (p<0.01, respectively p<0.05). The results of the this studyshowed that egg yolk addition to AhPlus do not interfere with its compounds, the data being in a close range withthose obtained by using the standard Lactose Egg Yolk extender (p>0.05). The combination egg yolk-AhPlus seemsto be an alternative to standard extenders, conferring stability in boar sperm cells against cold shock.
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