with bovine serum albumin as a standard. Then, 20 mg of the protein sample was loaded into polyacrylamide
gel, which consisted of 10% running gel and 4% of stacking gel. Electrophoresis was performed at constant current of
15 mA per gel, using a Mini Protein II unit (Bio-Rad Laboratories).The proteins were stained in Coomassie Brilliant Blue R-250. The excess strain was washed out by destaining the gel with a solution of methanol and glacial acetic acid.