The membranes could meet the properties needs of a wound dressing such as water absorption, water vapor transmission rate and cell proliferation. Therefore, with ADA adding into CS/SF membranes, it could promote cell proliferation and enhance the stability of membranes. Further investigation would be more precisely delineated by designing a suitable ADA fixed SF/CS membrane according to the in vivo study. The ADA fixed SF/CS blending membranes would be a commercial dressing for wound healing in the further clinic application.