Algae biomass is a potential raw material for the production of biofuels and other chemicals. In this study,
biomass of the marine algae, Ulva lactuca, Gelidium amansii, Laminaria japonica, and Sargassum fulvellum,
was treated with acid and commercially available hydrolytic enzymes. The hydrolysates contained glucose,
mannose, galactose, and mannitol, among other sugars, at different ratios. The Laminaria japonica
hydrolysate contained up to 30.5% mannitol and 6.98% glucose in the hydrolysate solids. Ethanogenic
recombinant Escherichia coli KO11 was able to utilize both mannitol and glucose and produced 0.4 g ethanol
per g of carbohydrate when cultured in L. japonica hydrolysate supplemented with Luria–Bertani
medium and hydrolytic enzymes. The strategy of acid hydrolysis followed by simultaneous enzyme treatment
and inoculation with E. coli KO11 could be a viable strategy to produce ethanol from marine alga
biomass.
Algae biomass is a potential raw material for the production of biofuels and other chemicals. In this study,biomass of the marine algae, Ulva lactuca, Gelidium amansii, Laminaria japonica, and Sargassum fulvellum,was treated with acid and commercially available hydrolytic enzymes. The hydrolysates contained glucose,mannose, galactose, and mannitol, among other sugars, at different ratios. The Laminaria japonicahydrolysate contained up to 30.5% mannitol and 6.98% glucose in the hydrolysate solids. Ethanogenicrecombinant Escherichia coli KO11 was able to utilize both mannitol and glucose and produced 0.4 g ethanolper g of carbohydrate when cultured in L. japonica hydrolysate supplemented with Luria–Bertanimedium and hydrolytic enzymes. The strategy of acid hydrolysis followed by simultaneous enzyme treatmentand inoculation with E. coli KO11 could be a viable strategy to produce ethanol from marine algabiomass.
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