In another study of healthy nondepressed individuals, 44 participants were given 400 IU per day,800 IU per day , or no in Vitamin D3 for 5 day during late winter in a randomized , double-blind, placebo-controlled study .The positive and Negative affect schedule was use as a self-report measure of positive and Negative affectivity. Results on this self-report measure showed that Vitamin D3 at both dosages significantly enhanced positive affect, with a non-significant trend toward a reduction in Negative affect