Most of the Microorganism has developed resistance to the existing antibiotics so it has provoked the need of a constant research on production of newer antibiotics in order to overcome the resistant microorganism. Antibiotics, because of their industrial importance, are the best known products of actinomycetes. The primary objective was to isolate the actinomycetes which having capacity to produce new antibiotic and characterize it. Soil sample collected from different place than tested their microbial sensitivity against different strains of bacteria. Initially 13 strains of microorganism were isolated. Out of 13 strains only 6 were selected based on their antibacterial activity. After than did taxonomical studies which confirmed the typical biochemical behaviors resembling actinomycetes. These tests helped to design the fermentation medium for antibiotic production. The strain A5 was selected based on its significant broad spectrum antibacterial activity against both Gram positive and Gram negative bacteria. This strain was further studied for it’s morphological, microscopically and cultural characteristics for classification of its taxonomical identification. From morphological and taxonomical studies, it was observed that the strain A5 was considered to belong to the Genus: Actinomyces