It is therefore logical to try to produce, on-site, tailor-made
enzymatic mixtures that are optimized for the specific pretreated
feedstock to be processed. Furthermore, the use of the same material
for enzyme production and hydrolysis could reduce the production
costs of second-generation ethanol, since both processes
could be co-located and share infrastructure and utilities. Moreover,
cultivation of the microorganisms using the same lignocellulosic
material that is intended to be hydrolyzed could be a way of
selecting for the production of enzymes that are optimal for the
hydrolysis of that specific material