Fig. 1. Screening of histamine-forming (HmF) strains isolated from fish nukazuke products. (A) Image of the screening. The isolates from fish nukazuke were inoculated into
0.5% histidine GAM broth containing 10% NaCl and incubated at 30 C for 4 days. HmF: positive strains; w: weak positive strain. The other wells show the results of negative
(HmN) strains. (B) Effect of NaCl on Hm production of HmF-strains. The strains were inoculated into 0.5% histidine GAM broth with ( ) or without (h) 10% NaCl, and were
incubated at 30 C for 4 days. Values are expressed as mean ± SD (n = 3).