PCR was performed after preliminary heating
at 94∘C for 2min according to the following scheme: 34
cycles of 15 s at 94∘C, 30 s at 55∘C, and 45 s at 72∘C and final
synthesis for 2min at 72∘C. The PCR products were analyzed
by electrophoretic separation in 2% agarose gel.