The preparative HPLC instrument used in this study was a preparative Agilent 1100 HPLC Series. Separation was carried out on this system with an Inerstil
Prep ODS column (20 mm 250 mm, 10 lm). MeOH–H2O
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(85:15) was used as a mobile phase with flow rate 2 mL min1. Analytical thin-layer chromatography (TLC) was performed with silica gel 60 F254, 0.25 mm