Where endocrine organ toxicity occurs, much of the initial diagnosis still remains with
the histopathologist and the recognition of change in organ mass and appearance.
Additionally, clinical observations such as fluid intake, lethargy etc. can be useful, and
effects on growth or reproductive function can be important clues in endocrine
toxicology. The listed methods 3 to 7 are generally selected when further characterization
of the mechanistic effect is required following earlier studies, or where the structure of
the compound strongly suggests a possible toxic effect. Some hormone measurements
may be included in toxicology studies of therapeutic agents which are directed at
alterations of endocrine metabolism