The Spirulina sp. was maintained in Spirulina Media at 28 C using 3000 lx light intensity. After a 21 day cultivation
period cells were harvested by centrifugation and were washed several times with deionised water in order to remove culture media and were kept on a filter paper to reduce the water content. The biomass was dried at 60 C in an oven for 24 h and milled to a gritty consistency. The biomass was sieved for particle sizes smaller than 1 mm and stored in a dark bottle and kept in a dry cabinet for experiments. All of the media are sterilized by autoclaving at 121 C for 20 min.