Both essential oils isolated from Turkish and Indian lemon
fruits samples were evaluated for antimicrobial activity
through disc diffusion method Each
essential oil was subjected to serial dilution by using dimethyl
sulphoxide (DMSO) as a solvent to give concentrations 2mg/ml,
1 mg/ml, 0.5 mg/ml, and 0.25 mg/ml solutions (Ahlam et al.,
2013). Amoxicillin was used as a standard for this study at the
concentration at 1 mg/ml. Each concentration of essential oil
was tested for their antimicrobial activity against one gram (þ)
bacteria (S. aureus) and three gram() bacteria (E. coli, P. vulgaris
and P. aeruginosa) on nutrient agar plates. Whatmann No. 1
sterile filter paper discs (6 mm diameter) were impregnated
with each concentration of both essential oils obtained from
Turkish and Indian lemon fruits samples and placed on the
inoculated agar. Amoxicillin standard was also used for this
study without essential oil on the inoculated agar. After inoculated,
all the plateswere incubated at 37 C for 24 h. Evaluation
of antibacterial activity was measured showing the diameter of
the zones of inhibition against the tested bacteria. Eachmethod
in this experiment was replicated three times.