2.7. Statistics
Experiments were performed in triplicate and each set of yields was averaged. The averages and Duncan t-test were computed to measure variations in the total oil yield extracted at various condi- tions from the different parts of Indian mackerel and also to mea- sure the variations in FA constituents of the oil yield using various techniques. The least significant difference at the 95% confident le- vel was calculated for each parameter. The maximum standard deviations were used as the basis for the error bars shown in Fig. 1.