Twelve animals of
varying sizes were weighed on a top loading balance (College®:
accuracy=0.01 g) and placed in individually in respirometer flasks.
The animals were then covered with a small piece of plastic mesh to
prevent movement into the side arm of the flask, which contained
approximately 0.5 mL of 20% KOH solution. The flasks were then
attached to the respirometer and inserted into a water bath at the
experimental temperature and allowed a 15 min acclimation period.