The control consisted of drying with sunlight. Soil tilling was performed using a shovel once every 7 days in treatments 1, 2 and 5. Therefore, soil was mixed with organic sludge during the tilling process. In treatments 2 and 3, 60 g of lime was added at the beginning of the experiment. Probiotic at a volume of 50 mL was sprayed on the pond bottom soil containing organic sludge in treatments 4 and 5. Total heterotrophic bacteria (THB) and pathogenic bacteria (Vibrio and Pseudomonas) were routinely determined once every 3 days during the experiment for the first 15 days.