The peel fractions of lemon (C. limonum) demonstrated
higher H-donor activity than the pulp fractions. Thus peel
fractions were better free radical scavenging constituents. The
readings were taken in triplicates and the average was
determined. When this odd electron gets paired up while a
hydrogen donor is present, then it decreases the absorption
strength. And this absorbance goes on decreasing, as the
antioxidants increase. In ethanol solution, DPPH produces a
violet colour. This resulting decolorization depends on the
number of electrons that are captured. With the addition of
pulp and peel fractions in a concentration-dependant
manner, the DPPH will give a yellow colour product known
as diphenylpicryl hydrazine, if the scavenging of free radicals
takes place. This scavenging occurs due to the donation of
hydrogen ions as a result of progression of the reaction
between free radicals and antioxidants. The number of
molecules of DPPH which are reduced could be co-related with
the hydroxide groups that are available. All the fractions
showed significant higher inhibition percentage i.e. stronger
hydrogen-donating ability