Nodal explants of mulberry (Morus alba L.), bearing an axillary bud, and obtained from in vitro raised seedlings were used to initiate shoot cultures. Murashige and Skoog medium supplemented with 3% sucrose and 2.5 μM N6-benzyl adenine resulted in a maximum rate of shoot multiplication (up to 13-fold) as expressed in terms of the number of nodes (propagules) formed per shoot. Nodal segments from 3–4-year-old greenhouse-grown plants behaved similarly. Nodal explants from different seedlings exhibited highly significant differences in the rate of shoot multiplication. In addition, the position of the nodal segment on the parent shoot determined the rate of shoot multiplication, with a basipetally increasing gradient in the growth response of the segments being observed. The cultured nodal segments could be cold stored at 4°C for at least 6 months without any loss of multiplication rate on subsequent culture at 25°C. The in vitro formed shoots underwent spontaneous rooting, after being on shoot proliferation medium for more than 4 weeks. Addition of activated charcoal (0.05 or 0.1%) to the shoot proliferation medium speeded up the production of adventitious roots by at least 2 weeks and inhibited the formation of callus at the basal end of the shoots. The rooted shoots could be transplanted to sand-vermiculite (1:1) mixture in the greenhouse with 100% transplantation success.