In order to study the in vitro callus induction and plant regeneration in the asparagus plant (Asparagus officinalis ) and
indirect organogenesis in explants, an experiment in a completely randomized design was performed in tissue culture
laboratory of faculty of agriculture, Islamic Azad University of Dezfool. In this study, the nodal explants treated with
different concentrations of NAA and BAP, and it was found that the presence of two plant growth regulators NAA And BAP
Is necessary for seedlings regeneration and callus induction in asparagus,. Treatment that leads to achieving the maximum
amount of callus buds was MS medium supplied with 0.5 mg/lit of NAA and 1 mg/lit BAP which leads to the production of 22
buds per explants. Two different levels of 2, 4-D were used for induce roots in regenerated seedlings. The best treatment was
1.25 mg/ lit 2, 4-D with an average of 52% rooting in regenerated plants.