2. Materials and methods
2.1. Material
The grains of C. album are not commonly available in commercial
market. Hence, the grains of C. album var. (Local Punjab) grains
were brought from local farmers of district Sangrur, Punjab (India).
Grains were cleaned and stored in air tight container in refrigeration
conditions at 4 C till further used. Wheat flour, shortening,
sugar, skim milk powder and salt were purchased from local market
Sangrur, (India).
2.2. Germination of C. album grains
Grains were soaked in distilled water (1:5) ratio, till their
saturation point was reached, excess water was drained and grains
were germinated in a Seed Germinator (Macro Scientific Works Pvt.
Ltd, India. Relative humidity ¼ 85%) with pre-standardized conditions
i.e. 25 C for 48 h. After germination the seeds were dried in
hot air oven at 45 C for 18 h till final moisture content of 9.75 g/
100 g. The dried grains both raw and germinated were milled in
stone mill and passed through 44 mesh sieve (355 mm) to obtain a
uniform size of flour. The flour was stored in refrigeration (4 C) till
further analysis.