Ribonucleases (RNase) are important analytical
enzymes and widely distributed in nature. RNase from
microorganisms widely used in molecular biology study, food,
and pharmaceutical industry. As an important analytical tool,
they have played a major role in study on the structure and
function of RNA. In single cell protein production, they are used
to remove RNA in cell. RNases have also been applied
commercially to produce nucleotides for clinical use or for
seasoning nucleotides. Many ribonucleases are highly cytotoxic.
They have several important biological roles such as antitumor
and antiviral activity.The selected strain Aspergillus niger
ATCC 26550 is chosen for extra cellular RNase enzyme
production by maintaining the culture medium at 30°C, pH 5.5
where the enzyme production was found to be growth
associated. Beef extract, peptone and ammonium sulfate are the
superior nitrogen source. Addition of Mg2+, Ca2+ to the growth
medium significantly enhanced the enzyme production. Glucose
found to be the excellent carbon source for RNase production.
This method of process optimization is the classical approach of
one factor at a time.