3.4. HPLC and UPLC chromatograms of flavones in LHs
The optimal chromatographic condition was obtained from
different mobile phases on an Ascentis C18 HPLC column
(250 mm 4.6 mm, 5 mm; Supelco Analytical) for HPLC, and on
a Waters ACQUITY UPLC BECH C18, 1.7 mm at 350 nm,
respectively. As shown in Fig. 3, the flavonoid profile can be
well separated under the current HPLC (I), and UPLC (II), conditions.
Quercitrin was used as an I.S. to monitor the reproducibility
and minimize the artificial error, which exhibited an
excellent separation from all analytes. HPLC analysis was
used for the following quantitative measurement of five major
components, 1e4, and 8.