Previous toxicological studies have shown that rats exposed to TBT display a drastically altered hormonal signature [3,24,53]. Nakanishi et al. [90] reported an increase of aromatase activity (AA) and estrogen production in human placental choriocarcinoma cells after TBT treatment for 48 h (0, 1, 10 and 100 nM). Our results agree with previous findings; the treatment with OTs in seafood increased serum estrogen levels in female rats. However, the effects of OTs in ovarian steroidogenesis are controversial. Grote et al. [24] found that TBT decreased ovarian AA and increased estrogen levels