2.3.2. Determination of minimum inhibitory and
bactericidal concentrations
The minimal inhibitory concentration (MIC) values were
determined by microdilution assay. This experiment was performed
by the method of Sahin et al. (2003). The cultures
were prepared at 24 h and 72 h broth cultures of Staphylococcus
epidermidis and Propionibacterium acnes, respectively.
The MIC was defined as the lowest concentration of
the compound to inhibit the growth of microorganisms and