All animals were kept under appropriate high-containment conditions and received a commercial feed for pigs of their age class once a day combined with hay cobs. Access to water was provided ad libitum. All applicable animal welfare regulations and standards were taken into account (permission with reference number 7221.3-1.1-018/12).The experimental setup including the sampling scheme is depicted in Table 1. Intramuscular injections were carried out deep into the muscles behind the ear using a 2 ml syringe and a 20 G needle.For the duration of the trial, rectal body temperature was measured on a daily basis. Fever was defined as a body temperature≥40.0◦C for at least two consecutive days. Clinical signs were recorded daily. Euthanasia was performed based on a humane endpoint system to prevent unacceptable suffering. At the end of the trial, all animals were subjected to post-mortem examinations.