is, the differences between corrected and uncorrected values were
only ca. 5%). Consequently, we consider the effect of null alleles on
our data to be very weak.
According to Evanno’s approach, K = 2, 9, 7 and 11 were the
most likely numbers of genetic clusters for Structure simulations
(Supplementary Fig. S1). Since K = 2 was not informative, we
focused on the result for K = 9, which was the second most likely
number of clusters (Fig. 2). Notably, in all the runs performed,
almost all the clusters generated corresponded to individual populations
or to taxa: (1) the yellow cluster to C. brunnea; (2) the
magenta cluster to populations CHR3 and CHR4 of C. chrysocephala;
(3) the green cluster to populations CHR5 and CHR2 of C. chrysocephala;
(4) the pale blue cluster to C. heldreichii; (5) the purple cluster
to C. litochorea; (6) the dark blue cluster to C. messenicolasiana;
(7) the salmon pink to population PRI2 of C. princeps; (8) and (9)
orange and red clusters to populations DEU1 and DEU2 of C. deusta,
respectively. Populations CHR1 of C. chrysocephala and PRI1 of C.
princeps, by contrast, showed an admixture of individuals from distinct
clusters. The results of Barrier based on Monmonier’s algorithm
(Supplementary Fig. S2) closely agreed with the genetic
patterns recovered using Structure. Most populations were separated
from the first assigned barrier, and the significance of the
separations (as measured by bootstrap values) increased with the
addition of new barriers, reaching the maximum values at 10
barriers.
is, the differences between corrected and uncorrected values wereonly ca. 5%). Consequently, we consider the effect of null alleles onour data to be very weak.According to Evanno’s approach, K = 2, 9, 7 and 11 were themost likely numbers of genetic clusters for Structure simulations(Supplementary Fig. S1). Since K = 2 was not informative, wefocused on the result for K = 9, which was the second most likelynumber of clusters (Fig. 2). Notably, in all the runs performed,almost all the clusters generated corresponded to individual populationsor to taxa: (1) the yellow cluster to C. brunnea; (2) themagenta cluster to populations CHR3 and CHR4 of C. chrysocephala;(3) the green cluster to populations CHR5 and CHR2 of C. chrysocephala;(4) the pale blue cluster to C. heldreichii; (5) the purple clusterto C. litochorea; (6) the dark blue cluster to C. messenicolasiana;(7) the salmon pink to population PRI2 of C. princeps; (8) and (9)orange and red clusters to populations DEU1 and DEU2 of C. deusta,respectively. Populations CHR1 of C. chrysocephala and PRI1 of C.princeps, by contrast, showed an admixture of individuals from distinctclusters. The results of Barrier based on Monmonier’s algorithm(Supplementary Fig. S2) closely agreed with the geneticpatterns recovered using Structure. Most populations were separatedfrom the first assigned barrier, and the significance of theseparations (as measured by bootstrap values) increased with theaddition of new barriers, reaching the maximum values at 10barriers.
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