T7-sRAGE cDNA optimization was performed with GeneOptimizer
expert software from Life Technologies-Invitrogen, using the
composite sequence provided by the authors. The sequence-optimized
composite T7-sRAGE sequence was then synthesized and
subcloned to a pcDNA 3.1(zeo+) vector between KpnI and XbaI
sites. Both the wild-type (WT) and the sequence-optimized sRAGE
(OP) were nucleotide-sequenced to ensure their correctness. A PDF
file containing both WT and OP T7-sRAGE is provided in Supplemental
material (SF1).