Experimental
Feedstock of Taihu algae bloom
The biomass of Taihu algae bloom was collected from Yangwan
Separation Station in Wuxi City, Jiangsu Province, China.
Morphology analysis revealed that Microcystis wesenbergii and
Microcystis aeruginosaare are the dominant species and account
for 40%e98% of algae blooms in Taihu Lake [8,9]. Afterward, the
biomass was subjected to air-flotation separation, dried under
the sun, milled, and stored for further experiments. Moisture
content was measured by drying in oven at 105 C until constant
mass was achieved. The contents of TVS and ash were
determined by heating at 600 C for 2 h. Protein content was
determined by Lowry method [10]. Total carbohydrates, lipids,
and heating value were determined as described in our previous
study [11,12]. Metal content was measured by flame atomic
absorption spectrophotometry (Thermoelectric SOLAR UNICAM
969 Atomic Absorption Spectrophotometer, USA). The
microcosmic structure of Taihu blue algae was observed using
SEM (Tabletop Microscope TM-1000, HITACHI) and TEM
(Tabletop Microscope H-7650, HITACHI).