The present study aims to investigate particular effects of
plant growth regulators such as BAP and NAA to establish
micropropagation protocols and ideal requirements for production
of coloured callus of E. cinerascens. Parameters
examined were the mean number of shoots per explant,mean (%) explants producing shoots and callus, mean shoot
height and diameter, pigment detection and analysis of
coloured callus. To the best of our knowledge, so far no
research paper was published on regeneration of this species
in vitro. In addition, analysis of coloured callus produced
in vitro has not been evaluated as yet. Only a few studies
reported on basic micropropagation of this genus and a
chemical study discovered this specific species contained
phenethylamines (Bruhn and Sa´nchez-Mejorada 1977).
Nowadays, our intention is directed not only to produce
plants on a large scale but also to reveal and introduce
multipurpose plants which could give benefits to human
kind. Further studies on detection of natural pigments in
coloured callus production may contribute valuable profits
in many industries, not only in agriculture but also in
medicine, cosmetics or even the food industry.