production, resulting on the decrease of the amount of BC
produced decrease for higher concentrations of (NH4)2SO4 and
KH2PO4. It is generally known that oxygen is critical in BC
production process [35] but these results show that nitrogen
and phosphorus sources could also play important roles and
should be taken into account for a subsequent process optimization.
However, more studies on the influence of these
two nutrients and a reliable method for immediate measurement
of Gluconacetobacter saccahri growth are required and
should be developed before attempting process optimization