By using high-performance liquid chromatography, it was found that a high-molecular-mass fraction of EPS included N-acetylglucosamine (54.4%) andglucuronic acid (45.6%), which are components of HA.
Furthermore, 13C-nuclear magnetic resonance spectroscopy showedthat the spectra of the high-molecular-mass fraction corresponded well to those of commercial HA.
Here, we described forthe first time that S. thermophilus, which is a generally recognized safe bacterium, produces HA.
The novel HA-producing bacterium S thermophilus YIT 2084 has great potential for applications in the medical, cosmetic and food fields, although its culture conditions remain to be improved.
By using high-performance liquid chromatography, it was found that a high-molecular-mass fraction of EPS included N-acetylglucosamine (54.4%) andglucuronic acid (45.6%), which are components of HA. Furthermore, 13C-nuclear magnetic resonance spectroscopy showedthat the spectra of the high-molecular-mass fraction corresponded well to those of commercial HA. Here, we described forthe first time that S. thermophilus, which is a generally recognized safe bacterium, produces HA. The novel HA-producing bacterium S thermophilus YIT 2084 has great potential for applications in the medical, cosmetic and food fields, although its culture conditions remain to be improved.
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