Pigment Analysis
All samples were extracted in acetone. The pigments
were extracted from C. officinalis flower with 80% acetone.
We used the specific absorption coefficients of
chlorophylls a and b, and carotenoids (Car) to estimate
their levels using spectrophotometry. All of the chlorophylls
contained two main absorption bands of radiations:
one on the blue side or near the UV portion of
the electromagnetic spectrum, and the other on the red
side or near the IR region of the electromagnetic spectrum.
The lack of a significant absorption on the green
side of the spectrum confers on chlorophylls their characteristic
green or blue-greenish colors.