A quick and inexpensive sample treatment, based on a singlestepmicroextraction with a SUPRAS made up of vesicles of octanoicacid, has been developed for the determination of PAH4 infoodstuffs belonging to different categories (smoked meat and fish,bivalve mollusks, and processed cereal-based food for infants) andfeaturing fat (0.3–12.1%), protein (2.5–18.5%) and carbohydrate(<1–12.4%) contents in a wide range. The two structure-derivedextraction properties of SUPRAS, namely different polarity regionsable to provide several types of interactions, and high number ofbinding sites, render SUPRAS excellent candidates to consider themas general extractants in food analysis.Valuable assets of the matrix-independent sample treatmenthere developed are: (1) it takes about 20 min for sample stirringand centrifugation; (2) SUPRAS preparation for extracting 30 foodsamples only requires mixing the ingredients and centrifugationfor 5 min; (3) ten samples can simultaneously stirred and 36 samplessimultaneously centrifuged with the vortex-shaker and centrifugeused in this research; (4) the method requires a low sampleamount (0.2 g), which is made representative of the bulk byhomogenization, and a low eco-friendly SUPRAS volume(200 lL); (5) conventional equipment in labs is used and (6) it featureslow cost [i.e. the cost of sample treatment per sample unit,only considering the consumption of volume of solvents, SPE cartridges,etc. was estimated to be 0.32 € in our method and 1.14 €(Ramalhosa et al., 2009), 1.83 € (Windal et al., 2008), 2.26 €(Forsberg et al., 2011), 2.58 € (Berntssen et al., 2010), 5.59 €(Rey-Salgueiro et al., 2009), 7.18 € (Farhadian et al., 2010) and18.03 € (Jira, 2010) in some previous reportedmethods]. Themethodproposed here can be used for the routine control of PAH4 in foodbelow the tolerance level permitted by the EU directives for BaP.
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