The embryogenic callus was induced from hypocotyls on MS medium within two weeks of culture, 1.0 mg l-1 2, 4-D being the most effective treatment in producing friable embryogenic callus (Fig. 1A) that were maintained well on medium added with other PGRs as well. The leaf, root and stem explants also produced calli but was turned out to be non-embryogenic.
The embryogenic callus was white yellow, friable and grew very fast on fresh nutrient medium upon regular sub-culturing.