The quality grades
of the cattle were A2–A3 according to Japanese carcass grading standards
and the intramuscular fat content was 7.6% ± 1.6%, which were
measured by the method of AOAC (1990). The cattle had a rest at least
for 12 h before stunning, and were slaughtered by Halal method.
Carcass hanging method was changed at the end of the slaughter
line within 45 min postmortem.