Fig. 1. Nested-PCR assay for detecting S. agalactiae DNA in tissue experimentally inoculated
with S. agalactiae ST991 and naturally infected with S. agalactiae. Amplification of a 192 bp
fragment from the S. agalactiae 16S-23S rRNA intergene region. Lanes 1 and 2: frozen eyes
from experimentally inoculated fry; lanes 3 and 4: frozen brains from experimentally inoculated
fry; lane 5: 100 bp DNA ladder; lanes 6, 7, 8 and 9: samples of naturally infected fish
tissue; lane 10: S. agalactiae ST991 genomic DNA positive control; lane 11: negative control.