Table 1
Mobil phase gradient for determination of carotenoids by HPLC.
Time (min) Flow (mL/min) A (%) B (%) C (%)
0 1 80 0 20
5 1 77.5 0 22.5
22.5 1 50 0 50
27.5 1 50 0 50
30 1 20 0 80
35 1 20 0 80
40 1 0 0 100
45 1 0 0 100
50 1 0 100 0
70 1 0 100 0
71 1 80 0 20
80 1 80 0 20
A: methanol/water (75:25). B: methanol (100%). C: ethyl acetate (10